MASS PHOTOMETER

Mass Photometry Notes

Buffer Solvent Recommendations (From Refeyn)

In order to optimize detection accuracy and resolution of lower molecular weight species and minimize background noise, particularly in the lower molecular weight range, the following steps are recommended. Using the highest quality DI water and buffer and the procedure below yields the best results

Materials

VWR: VWRL0201-0500 Water, Molecular Biology Grade 0.2um filtered

VWR: 82020-066 HyClone Dulbecco's Phosphate Buffered Saline Solution

Aliquot stock solutions into 15 ml sterile conical tubes by pouring (do not use pipets)

Freeze stock aliquots until use.

On the day of use, thaw the required number of stock tubes and aliquot into 1.5 ml microcentrifuge tubes.

Using sterile tips and storing tubes on ice may minimize bacterial growth. Discard unused solutions after one day.